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pet-15b vector  (Millipore)


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    Structured Review

    Millipore pet-15b vector
    Pet 15b Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet-15b+vector/pet28a/us12059418-269-19-21
    Average 90 stars, based on 1 article reviews
    pet-15b vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: Different RNA recognition by ProQ and FinO depends on the sequence surrounding intrinsic terminator hairpins
    Article Snippet: To obtain the FinO protein the overexpression construct was prepared by cloning the coding sequence of finO into pET-15b vector (Novagen) using BamHI restriction site (Supplemental Table S2).

    Article Title: Different RNA recognition by ProQ and FinO depends on the sequence surrounding intrinsic terminator hairpins
    Article Snippet: The sequences of Escherichia coli ProQ protein, and its 130-aa long N-terminal domain were cloned into pET-15b vector (Novagen), and purified as previously described ( ).

    Article Title: Cryo-EM Structure of AAV2 Rep68 bound to integration site AAVS1: Insights into the mechanism of DNA melting
    Article Snippet: All proteins were expressed using a modified pET-15b vector, expressed in E. coli BL21(DE3) cells (Novagen), and purified as described before [ ].

    Article Title: Plasmodial surface anion channel inhibitors for the treatment or prevention of malaria
    Article Snippet: Polyclonal Antibody Production DNA sequence encoding the C-terminal 141 amino acids of the Dd2 clag3.1 product was cloned into pET-15b vector (Novagen) for over-expression in E. coli.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1
    Article Snippet: The DNA fragments encoding Arabidopsis thaliana AtH2A.13, AtH2A.W.6, AtH3.1, and AtH4 were inserted into the pET-15b vector (Novagen).

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1.
    Article Snippet: Purification of recombinant Arabidopsis DDM1 The DNA fragment encoding AtDDM1 was inserted into the pET-15b vector (Novagen), in which the sequence of the thrombin recognition site (Leu-Val-Pro-Arg-Gly-Ser-His) was substituted with that of the human rhinovirus (HRV) 3C protease (Leu-Glu-Val-Leu-Phe-Gln-GlyPro), and a SUMO tagwas integrated between theHis6-tag and theHRV 3C protease recognition site.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1
    Article Snippet: The DNA fragment encoding AtDDM1 was inserted into the pET-15b vector (Novagen), in which the sequence of the thrombin recognition site (Leu-Val-Pro-Arg-Gly-Ser-His) was substituted with that of the human rhinovirus (HRV) 3C protease (Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro), and a SUMO tag was integrated between the His 6 -tag and the HRV 3C protease recognition site.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1.
    Article Snippet: Preparation and purification of the nucleosome containing Arabidopsis histones The DNA fragments encoding Arabidopsis thaliana AtH2A.13, AtH2A.W.6, AtH3.1, and AtH4 were inserted into the pET-15b vector (Novagen).

    Clone Assay:

    Article Title: Different RNA recognition by ProQ and FinO depends on the sequence surrounding intrinsic terminator hairpins
    Article Snippet: To obtain the FinO protein the overexpression construct was prepared by cloning the coding sequence of finO into pET-15b vector (Novagen) using BamHI restriction site (Supplemental Table S2).

    Article Title: Different RNA recognition by ProQ and FinO depends on the sequence surrounding intrinsic terminator hairpins
    Article Snippet: The sequences of Escherichia coli ProQ protein, and its 130-aa long N-terminal domain were cloned into pET-15b vector (Novagen), and purified as previously described ( ).

    Article Title: Cryo-EM Structure of AAV2 Rep68 bound to integration site AAVS1: Insights into the mechanism of DNA melting
    Article Snippet: All proteins were expressed using a modified pET-15b vector, expressed in E. coli BL21(DE3) cells (Novagen), and purified as described before [ ].

    Article Title: Plasmodial surface anion channel inhibitors for the treatment or prevention of malaria
    Article Snippet: Polyclonal Antibody Production DNA sequence encoding the C-terminal 141 amino acids of the Dd2 clag3.1 product was cloned into pET-15b vector (Novagen) for over-expression in E. coli.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1
    Article Snippet: The DNA fragments encoding Arabidopsis thaliana AtH2A.13, AtH2A.W.6, AtH3.1, and AtH4 were inserted into the pET-15b vector (Novagen).

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1.
    Article Snippet: Purification of recombinant Arabidopsis DDM1 The DNA fragment encoding AtDDM1 was inserted into the pET-15b vector (Novagen), in which the sequence of the thrombin recognition site (Leu-Val-Pro-Arg-Gly-Ser-His) was substituted with that of the human rhinovirus (HRV) 3C protease (Leu-Glu-Val-Leu-Phe-Gln-GlyPro), and a SUMO tagwas integrated between theHis6-tag and theHRV 3C protease recognition site.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1
    Article Snippet: The DNA fragment encoding AtDDM1 was inserted into the pET-15b vector (Novagen), in which the sequence of the thrombin recognition site (Leu-Val-Pro-Arg-Gly-Ser-His) was substituted with that of the human rhinovirus (HRV) 3C protease (Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro), and a SUMO tag was integrated between the His 6 -tag and the HRV 3C protease recognition site.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1.
    Article Snippet: Preparation and purification of the nucleosome containing Arabidopsis histones The DNA fragments encoding Arabidopsis thaliana AtH2A.13, AtH2A.W.6, AtH3.1, and AtH4 were inserted into the pET-15b vector (Novagen).

    Plasmid Preparation:

    Article Title: Different RNA recognition by ProQ and FinO depends on the sequence surrounding intrinsic terminator hairpins
    Article Snippet: To obtain the FinO protein the overexpression construct was prepared by cloning the coding sequence of finO into pET-15b vector (Novagen) using BamHI restriction site (Supplemental Table S2).

    Article Title: Different RNA recognition by ProQ and FinO depends on the sequence surrounding intrinsic terminator hairpins
    Article Snippet: The sequences of Escherichia coli ProQ protein, and its 130-aa long N-terminal domain were cloned into pET-15b vector (Novagen), and purified as previously described ( ).

    Article Title: Cryo-EM Structure of AAV2 Rep68 bound to integration site AAVS1: Insights into the mechanism of DNA melting
    Article Snippet: All proteins were expressed using a modified pET-15b vector, expressed in E. coli BL21(DE3) cells (Novagen), and purified as described before [ ].

    Article Title: Plasmodial surface anion channel inhibitors for the treatment or prevention of malaria
    Article Snippet: Polyclonal Antibody Production DNA sequence encoding the C-terminal 141 amino acids of the Dd2 clag3.1 product was cloned into pET-15b vector (Novagen) for over-expression in E. coli.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1
    Article Snippet: The DNA fragments encoding Arabidopsis thaliana AtH2A.13, AtH2A.W.6, AtH3.1, and AtH4 were inserted into the pET-15b vector (Novagen).

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1.
    Article Snippet: Purification of recombinant Arabidopsis DDM1 The DNA fragment encoding AtDDM1 was inserted into the pET-15b vector (Novagen), in which the sequence of the thrombin recognition site (Leu-Val-Pro-Arg-Gly-Ser-His) was substituted with that of the human rhinovirus (HRV) 3C protease (Leu-Glu-Val-Leu-Phe-Gln-GlyPro), and a SUMO tagwas integrated between theHis6-tag and theHRV 3C protease recognition site.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1
    Article Snippet: The DNA fragment encoding AtDDM1 was inserted into the pET-15b vector (Novagen), in which the sequence of the thrombin recognition site (Leu-Val-Pro-Arg-Gly-Ser-His) was substituted with that of the human rhinovirus (HRV) 3C protease (Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro), and a SUMO tag was integrated between the His 6 -tag and the HRV 3C protease recognition site.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1.
    Article Snippet: Preparation and purification of the nucleosome containing Arabidopsis histones The DNA fragments encoding Arabidopsis thaliana AtH2A.13, AtH2A.W.6, AtH3.1, and AtH4 were inserted into the pET-15b vector (Novagen).

    Purification:

    Article Title: Different RNA recognition by ProQ and FinO depends on the sequence surrounding intrinsic terminator hairpins
    Article Snippet: To obtain the FinO protein the overexpression construct was prepared by cloning the coding sequence of finO into pET-15b vector (Novagen) using BamHI restriction site (Supplemental Table S2).

    Article Title: Different RNA recognition by ProQ and FinO depends on the sequence surrounding intrinsic terminator hairpins
    Article Snippet: The sequences of Escherichia coli ProQ protein, and its 130-aa long N-terminal domain were cloned into pET-15b vector (Novagen), and purified as previously described ( ).

    Article Title: Cryo-EM Structure of AAV2 Rep68 bound to integration site AAVS1: Insights into the mechanism of DNA melting
    Article Snippet: All proteins were expressed using a modified pET-15b vector, expressed in E. coli BL21(DE3) cells (Novagen), and purified as described before [ ].

    Article Title: Plasmodial surface anion channel inhibitors for the treatment or prevention of malaria
    Article Snippet: Polyclonal Antibody Production DNA sequence encoding the C-terminal 141 amino acids of the Dd2 clag3.1 product was cloned into pET-15b vector (Novagen) for over-expression in E. coli.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1
    Article Snippet: The DNA fragments encoding Arabidopsis thaliana AtH2A.13, AtH2A.W.6, AtH3.1, and AtH4 were inserted into the pET-15b vector (Novagen).

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1.
    Article Snippet: Purification of recombinant Arabidopsis DDM1 The DNA fragment encoding AtDDM1 was inserted into the pET-15b vector (Novagen), in which the sequence of the thrombin recognition site (Leu-Val-Pro-Arg-Gly-Ser-His) was substituted with that of the human rhinovirus (HRV) 3C protease (Leu-Glu-Val-Leu-Phe-Gln-GlyPro), and a SUMO tagwas integrated between theHis6-tag and theHRV 3C protease recognition site.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1
    Article Snippet: The DNA fragment encoding AtDDM1 was inserted into the pET-15b vector (Novagen), in which the sequence of the thrombin recognition site (Leu-Val-Pro-Arg-Gly-Ser-His) was substituted with that of the human rhinovirus (HRV) 3C protease (Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro), and a SUMO tag was integrated between the His 6 -tag and the HRV 3C protease recognition site.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1.
    Article Snippet: Preparation and purification of the nucleosome containing Arabidopsis histones The DNA fragments encoding Arabidopsis thaliana AtH2A.13, AtH2A.W.6, AtH3.1, and AtH4 were inserted into the pET-15b vector (Novagen).

    Over Expression:

    Article Title: Different RNA recognition by ProQ and FinO depends on the sequence surrounding intrinsic terminator hairpins
    Article Snippet: To obtain the FinO protein the overexpression construct was prepared by cloning the coding sequence of finO into pET-15b vector (Novagen) using BamHI restriction site (Supplemental Table S2).

    Article Title: Different RNA recognition by ProQ and FinO depends on the sequence surrounding intrinsic terminator hairpins
    Article Snippet: The sequences of Escherichia coli ProQ protein, and its 130-aa long N-terminal domain were cloned into pET-15b vector (Novagen), and purified as previously described ( ).

    Article Title: Cryo-EM Structure of AAV2 Rep68 bound to integration site AAVS1: Insights into the mechanism of DNA melting
    Article Snippet: All proteins were expressed using a modified pET-15b vector, expressed in E. coli BL21(DE3) cells (Novagen), and purified as described before [ ].

    Article Title: Plasmodial surface anion channel inhibitors for the treatment or prevention of malaria
    Article Snippet: Polyclonal Antibody Production DNA sequence encoding the C-terminal 141 amino acids of the Dd2 clag3.1 product was cloned into pET-15b vector (Novagen) for over-expression in E. coli.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1
    Article Snippet: The DNA fragments encoding Arabidopsis thaliana AtH2A.13, AtH2A.W.6, AtH3.1, and AtH4 were inserted into the pET-15b vector (Novagen).

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1.
    Article Snippet: Purification of recombinant Arabidopsis DDM1 The DNA fragment encoding AtDDM1 was inserted into the pET-15b vector (Novagen), in which the sequence of the thrombin recognition site (Leu-Val-Pro-Arg-Gly-Ser-His) was substituted with that of the human rhinovirus (HRV) 3C protease (Leu-Glu-Val-Leu-Phe-Gln-GlyPro), and a SUMO tagwas integrated between theHis6-tag and theHRV 3C protease recognition site.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1
    Article Snippet: The DNA fragment encoding AtDDM1 was inserted into the pET-15b vector (Novagen), in which the sequence of the thrombin recognition site (Leu-Val-Pro-Arg-Gly-Ser-His) was substituted with that of the human rhinovirus (HRV) 3C protease (Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro), and a SUMO tag was integrated between the His 6 -tag and the HRV 3C protease recognition site.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1.
    Article Snippet: Preparation and purification of the nucleosome containing Arabidopsis histones The DNA fragments encoding Arabidopsis thaliana AtH2A.13, AtH2A.W.6, AtH3.1, and AtH4 were inserted into the pET-15b vector (Novagen).

    Construct:

    Article Title: Different RNA recognition by ProQ and FinO depends on the sequence surrounding intrinsic terminator hairpins
    Article Snippet: To obtain the FinO protein the overexpression construct was prepared by cloning the coding sequence of finO into pET-15b vector (Novagen) using BamHI restriction site (Supplemental Table S2).

    Article Title: Different RNA recognition by ProQ and FinO depends on the sequence surrounding intrinsic terminator hairpins
    Article Snippet: The sequences of Escherichia coli ProQ protein, and its 130-aa long N-terminal domain were cloned into pET-15b vector (Novagen), and purified as previously described ( ).

    Article Title: Cryo-EM Structure of AAV2 Rep68 bound to integration site AAVS1: Insights into the mechanism of DNA melting
    Article Snippet: All proteins were expressed using a modified pET-15b vector, expressed in E. coli BL21(DE3) cells (Novagen), and purified as described before [ ].

    Article Title: Plasmodial surface anion channel inhibitors for the treatment or prevention of malaria
    Article Snippet: Polyclonal Antibody Production DNA sequence encoding the C-terminal 141 amino acids of the Dd2 clag3.1 product was cloned into pET-15b vector (Novagen) for over-expression in E. coli.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1
    Article Snippet: The DNA fragments encoding Arabidopsis thaliana AtH2A.13, AtH2A.W.6, AtH3.1, and AtH4 were inserted into the pET-15b vector (Novagen).

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1.
    Article Snippet: Purification of recombinant Arabidopsis DDM1 The DNA fragment encoding AtDDM1 was inserted into the pET-15b vector (Novagen), in which the sequence of the thrombin recognition site (Leu-Val-Pro-Arg-Gly-Ser-His) was substituted with that of the human rhinovirus (HRV) 3C protease (Leu-Glu-Val-Leu-Phe-Gln-GlyPro), and a SUMO tagwas integrated between theHis6-tag and theHRV 3C protease recognition site.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1
    Article Snippet: The DNA fragment encoding AtDDM1 was inserted into the pET-15b vector (Novagen), in which the sequence of the thrombin recognition site (Leu-Val-Pro-Arg-Gly-Ser-His) was substituted with that of the human rhinovirus (HRV) 3C protease (Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro), and a SUMO tag was integrated between the His 6 -tag and the HRV 3C protease recognition site.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1.
    Article Snippet: Preparation and purification of the nucleosome containing Arabidopsis histones The DNA fragments encoding Arabidopsis thaliana AtH2A.13, AtH2A.W.6, AtH3.1, and AtH4 were inserted into the pET-15b vector (Novagen).

    Recombinant:

    Article Title: Different RNA recognition by ProQ and FinO depends on the sequence surrounding intrinsic terminator hairpins
    Article Snippet: To obtain the FinO protein the overexpression construct was prepared by cloning the coding sequence of finO into pET-15b vector (Novagen) using BamHI restriction site (Supplemental Table S2).

    Article Title: Different RNA recognition by ProQ and FinO depends on the sequence surrounding intrinsic terminator hairpins
    Article Snippet: The sequences of Escherichia coli ProQ protein, and its 130-aa long N-terminal domain were cloned into pET-15b vector (Novagen), and purified as previously described ( ).

    Article Title: Cryo-EM Structure of AAV2 Rep68 bound to integration site AAVS1: Insights into the mechanism of DNA melting
    Article Snippet: All proteins were expressed using a modified pET-15b vector, expressed in E. coli BL21(DE3) cells (Novagen), and purified as described before [ ].

    Article Title: Plasmodial surface anion channel inhibitors for the treatment or prevention of malaria
    Article Snippet: Polyclonal Antibody Production DNA sequence encoding the C-terminal 141 amino acids of the Dd2 clag3.1 product was cloned into pET-15b vector (Novagen) for over-expression in E. coli.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1
    Article Snippet: The DNA fragments encoding Arabidopsis thaliana AtH2A.13, AtH2A.W.6, AtH3.1, and AtH4 were inserted into the pET-15b vector (Novagen).

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1.
    Article Snippet: Purification of recombinant Arabidopsis DDM1 The DNA fragment encoding AtDDM1 was inserted into the pET-15b vector (Novagen), in which the sequence of the thrombin recognition site (Leu-Val-Pro-Arg-Gly-Ser-His) was substituted with that of the human rhinovirus (HRV) 3C protease (Leu-Glu-Val-Leu-Phe-Gln-GlyPro), and a SUMO tagwas integrated between theHis6-tag and theHRV 3C protease recognition site.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1
    Article Snippet: The DNA fragment encoding AtDDM1 was inserted into the pET-15b vector (Novagen), in which the sequence of the thrombin recognition site (Leu-Val-Pro-Arg-Gly-Ser-His) was substituted with that of the human rhinovirus (HRV) 3C protease (Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro), and a SUMO tag was integrated between the His 6 -tag and the HRV 3C protease recognition site.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1.
    Article Snippet: Preparation and purification of the nucleosome containing Arabidopsis histones The DNA fragments encoding Arabidopsis thaliana AtH2A.13, AtH2A.W.6, AtH3.1, and AtH4 were inserted into the pET-15b vector (Novagen).

    Modification:

    Article Title: Different RNA recognition by ProQ and FinO depends on the sequence surrounding intrinsic terminator hairpins
    Article Snippet: To obtain the FinO protein the overexpression construct was prepared by cloning the coding sequence of finO into pET-15b vector (Novagen) using BamHI restriction site (Supplemental Table S2).

    Article Title: Different RNA recognition by ProQ and FinO depends on the sequence surrounding intrinsic terminator hairpins
    Article Snippet: The sequences of Escherichia coli ProQ protein, and its 130-aa long N-terminal domain were cloned into pET-15b vector (Novagen), and purified as previously described ( ).

    Article Title: Cryo-EM Structure of AAV2 Rep68 bound to integration site AAVS1: Insights into the mechanism of DNA melting
    Article Snippet: All proteins were expressed using a modified pET-15b vector, expressed in E. coli BL21(DE3) cells (Novagen), and purified as described before [ ].

    Article Title: Plasmodial surface anion channel inhibitors for the treatment or prevention of malaria
    Article Snippet: Polyclonal Antibody Production DNA sequence encoding the C-terminal 141 amino acids of the Dd2 clag3.1 product was cloned into pET-15b vector (Novagen) for over-expression in E. coli.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1
    Article Snippet: The DNA fragments encoding Arabidopsis thaliana AtH2A.13, AtH2A.W.6, AtH3.1, and AtH4 were inserted into the pET-15b vector (Novagen).

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1.
    Article Snippet: Purification of recombinant Arabidopsis DDM1 The DNA fragment encoding AtDDM1 was inserted into the pET-15b vector (Novagen), in which the sequence of the thrombin recognition site (Leu-Val-Pro-Arg-Gly-Ser-His) was substituted with that of the human rhinovirus (HRV) 3C protease (Leu-Glu-Val-Leu-Phe-Gln-GlyPro), and a SUMO tagwas integrated between theHis6-tag and theHRV 3C protease recognition site.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1
    Article Snippet: The DNA fragment encoding AtDDM1 was inserted into the pET-15b vector (Novagen), in which the sequence of the thrombin recognition site (Leu-Val-Pro-Arg-Gly-Ser-His) was substituted with that of the human rhinovirus (HRV) 3C protease (Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro), and a SUMO tag was integrated between the His 6 -tag and the HRV 3C protease recognition site.

    Article Title: Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1.
    Article Snippet: Preparation and purification of the nucleosome containing Arabidopsis histones The DNA fragments encoding Arabidopsis thaliana AtH2A.13, AtH2A.W.6, AtH3.1, and AtH4 were inserted into the pET-15b vector (Novagen).



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